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The results were correlated on the NGS-dependent abundance in the dominant butyrate-making genera Subdoligranulum
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Because various universal bacterial primers have been designed and optimized especially for qPCR or NGS, it can be sensible to take into account opportunity biases ensuing from primer-distinct amplification effectiveness for unique taxa [35] together with differential primer coverage, when utilizing different primer sets for qPCR and NGS. With this examine, we chose a broadly-used universal bacterial primer established optimized for qPCR [29] that similarly targets the V3-V4 hypervariable regions as being the primer established employed for Illumina MiSeq (S1A Table).
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